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We narrowed to 403 results for: mag

Showing: 141 - 160 of 403 results

  1. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ... run, all markers are read in parallel.  Let’s imagine we’re looking for cells in a sample that are negative...often easier to identify different populations by imagining (or drawing) a quadrant over the dot plot. Created...to see these populations, I suggest using the imaginary quadrant to help (Figure 5). Even though this ...

  2. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... for imaging applications; better tissue penetration which is useful for therapeutic and imaging applications...frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about antibodies  Read about another...

  3. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...virtually” via a webcam. In the office, we use a Black Magic Pocket Cinema Camera 4K. It’s wonderful and simple...zoom, stop/start record, and allow me to see an image.  Remote video: So first things first was getting...HDMI transmitter/receiver, to beam the camera’s image to my remote location, live. After doing a bit of...

  4. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...of pTOX that encode one of two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018...hours, the resulting colonies turn subtly blue or magenta. This is particularly valuable when first optimizing...antibiotic resistant clones. But if they are blue or magenta, they are real! Planning an allelic exchange experiment...

  5. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...updated by Susanna Stroik January 24, 2023. DNA damage drives genome instability and contributes to cancer...or it can introduce specific mutations into the damaged DNA if these modifications exist in the template...synthesized sequences are retained by the original damaged DNA molecule. Unlike in DSBR, following strand ...

  6. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...gutting 38 EPA programs: draft budget  Science Magazine: A grim budget day for U.S. science: analysis ...analysis and reaction to Trump’s plan Science Magazine: NIH, DOE Office of Science face deep cuts in Trump’s ...Environmental Science and Technology: Crossing The Imaginary Line The New York Times: A Scientists’ March on...

  7. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...looks like the one found on the left side of the image below. It has a promoter (blue arrow) followed by... using UV light – please note that UV light can damage DNA and that proper personal protective equipment... worn when visualizing using UV as it can cause damage to the eyes and skin. When running a gel for purification...

  8. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ... have expressed GFP) that I couldn’t get a good image. You could probably also do Western blotting against..., but to do it you would need a pretty good gel image. If you are analyzing short DNA fragments, run PAGE...Primers UCSC Genome Browser: For eploring genomic DNA ImageJ: Free Software for Analyzing Gels ...

  9. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...contain many epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always...concentration of cell lysate was added to each well. Image from Sun et al., 2010.   Enzyme-linked immunosorbent...fluorophore-conjugated secondary antibody or Fab. Image from Harden et al., 2014.   In vivo uses For ...

  10. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie Buss and Mengtong...Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv YanevCellosaurus: A Cell...

  11. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...  HuBMAP Consortium Healthy human cells Imaging (IHC, ICC, IF, etc.)  Data repository From ... Knowledge-Base  Any IBEX multiplex tissue imaging Data repository See blog post mAb3D Atlas...

  12. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...addgene.org to learn more. A great submission includes: Images that clearly show viral spread and cellular tropism... a functional vector such as a channelrhodopsin Images showing the injection site as well as the target...

  13. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...of 304 open access publishers. By the time the magazine went to press, no less than 157 of those journals...let us know your favorite ways to share science. Image credit: Judy Breck, via flickr  ...

  14. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...cells for ctrA gene expression using various dCas9. Image adapted from Guzzo et al., 2020. Caulobacter ... Figure 2: The components of Casilio-ME1. Image from Taghbalout et al., 2019. Fusing epigenetic...

  15. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...ColabFold (Mirdita et al., 2022). B, Representative images of HEK293T cells and neurons expressing nLightG...for Me?  In Living Color: The Skinny on In Vivo Imaging Tools Resources on Addgene.org Find Biosensor...

  16. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...highlights written as 8 tweetable facts. Note: The images used in this post were created using data from ...scientific domains tweeted by biologists were: Nature, Sciencemag, Wiley, Cell and Scientific American Figure ...

  17. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...of 1.2 per megabase in mammals), leading to DNA damage and developmental aberrations. In multiple systems...10.1038/ni0707-665. PubMed. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. ...

  18. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...source of mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al..../fimmu.2017.01589 Jin B, Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation, Diagnostics...

  19. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...routinely deposited in Addgene!” As you can imagine, answering plasmid requests can be a time-consuming...researchers from far corners of the world. “It’s easy to imagine how hard it is to distribute thousands of plasmids...

  20. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody...includes frequently cited antibodies for use in brain imaging (Andrews et al., 2019), validated in both western...
Showing: 141 - 160 of 403 results