We narrowed to 42 results for: mag

...Fluorescent Proteins In Vivo Imaging Fluorescent Proteins: In Vivo Imaging Far-Red Near-Infrared Photoactivatable...spatially selective imaging of tissues in living animals. Learn more about in vivo imaging tools in this blog...Find plasmids for in vivo imaging using far red, near-infrared, and photoactivatable fluorescent proteins...powerful tool to study proteins and cells, but imaging in deep-tissue organs or whole animals presents... fluorescent proteins most suitable for stable imaging in live animals are those with emission near or... brightness and allow for single or multicolor imaging. Photoactivatable iRFPs can be turned on by non-phototoxic...

...England Biolabs Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology...Krumm H, Johnsson K, Johnsson N. 2005. Multicolor imaging of cell surface proteins. J Am Chem Soc. 127(37...

...5.8 Monomer mAzami Green-N1 (mAG) - Mammalian Expression mAzami Green-C1 (mAG) - Mammalian Expression cfSGFP2...shift), useful for applications like multicolor imaging with better spectral separation. Protein Excitation...Choosing Your Fluorescent Proteins for Multi-Color Imaging FPbase (Link opens in a new window) : an open-source...

.... Takeharu Nagai Magnesium MagFRET-1 Mg 2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically...Tantama ATP QUEEN ATP sensors for live-cell imaging Live cell imaging of metabolic heterogeneity by quantitative...Mullineaux Insulin Sensor for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion in ...Ultrafast neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine dynamics...11985. Eckhard Boles pH Imaging intracellular pH in live cells (pHRed) Imaging Intracellular pH in Live... for imaging exocytosis and endocytosis (pHuji). Semisynthetic fluorescent pH sensors for imaging exocytosis...into cells, tissues, or organisms for long-term imaging and can be designed to specifically localize to...

...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...a substrate kit. Image the membrane using either X-ray film in a darkroom or an image capture machine....reaction can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...antibody, incubate, and wash. Preserve sample and image using a microscope. Special considerations Many ...

... in the near-infrared range, ideal for in vivo imaging. teLuc : Red-shifted bioluminescence when paired...provide more flexibility in emission colors and imaging without the need for an illumination source. BRET...BRET can be particularly useful for in vivo imaging or creating biosensors. Read more about BRET on our...Enhanced Nano-lanterns and Antares ) for in vivo imaging. NanoBRET : Constructs using NanoLuc® as a BRET...LSSmOrange-NanoLuc ( OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored...GPCR activity. AIMTOR : BRET biosensor for live imaging of mTOR signaling. Additional Resources Promega...

...repress target genes, purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA...antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9-VPR...Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including the simplicity...detect multiple targets simultaneously. CRISPR imaging can visualize chromatin and RNA dynamics in live...RNA-binding proteins (RBPs). Multicolor CRISPR imaging can be achieved by using orthogonal dCas9s (e.g... stability and amplifies the signal for better imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent...Cas13 fusions have also been used in in vivo RNA imaging or to control alternative splicing. Figure 13: ...

...targeted genomic loci. CRISPR imaging has numerous advantages over other imaging techniques, including that...cell imaging. Compared to techniques like fluorescence in situ hybridization (FISH), CRISPR imaging offers...

... read out the barcodes, each protein tag can be imaged with a corresponding primary and dye-conjugated...microscopy workflows to achieve high-resolution optical imaging down to individual synapses. Figure 2: PRISM workflow...unique combinations of protein bits. B) Multiplex imaging of barcodes with expansion microscopy and reconstruction...S.W., Damstra, H.G.J., Leible, D., Leung, K.S., Magno, C.A., Meeran, A., Michalska, J.M., Rieger, F., ...

...suppressor activated by cellular stresses like DNA damage, oxidative stress, and nutrient deprivation. p53...cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose from its observed molecular...Gadd45 GADD45A GADD45B GADD45G Growth arrest and DNA-damage-inducible; alpha, beta, or gamma IGF-BP3 Insulin-like...2A p21 Cyclin-dependent kinase inhibitor 1A p48 Damage-specific DNA binding protein 2, 48kDa p53 Tumor...

...aggregates in real time. Live imaging of astroctyes - Kelly Monk Lab. Live imaging tools to study the development...high-throughput screening and non-invasive live imaging. A standard toolbox of genetic methods, such as...Lipid droplet reporters - Richard White Lab. An imaging platform to study lipid dynamics in vivo. Bimolecular...

...well-characterized marker proteins. In Vivo Imaging Image with far-red or infrared probes to study molecular...Science Plasmid Collection New England BioLabs Cell-Imaging Plasmid Collection Promega Plasmid Collection Botman-Teusink...

... functional neural circuits at the growth cone. Image credit: IPI Browse Netrin, DCC, and IGDCC3 (PUNC...and postsynaptic membranes at neuronal synapses. Image credit: IPI Browse Neurexin and Neuroligin Antibodies...integrin function, and/or detect integrin expression. Image credit: IPI Browse Integrin Antibodies ID Recombinant...

...donor can be measured using fluorescence lifetime imaging microscopy (FLIM) or FLIM-FRET, where the increased...et al. (2005) Quantitative multiphoton spectral imaging and its use for measuring resonance energy transfer...and stoichiometry of protein interactions using image-based quantitative two-hybrid FRET Positive and ...

... sequence (COX8) mStayGold Janelia Integrative Imaging 189774 p4xmts-mScarlet3_N1 Mitochondria 4x Mitochondrial...Microtubules alpha-Tubulin mStayGold Janelia Integrative Imaging 231779 pCMV-mGold2t-Tubulin-C-18 Microtubules alpha-Tubulin... Lysosomes Lamp1 mStayGold Janelia Integrative Imaging 231778 pCMV-mGold2t-Lysosome-N-20 Lysosomes Lamp1...

...-95 Human Mouse IgG2a 177440 Anti-Pan-MAGUK [K28/86R] Pan-MAGUK Human Mouse IgG2a 177441 Anti-Kvbeta1.2...TrpC6 Human Mouse IgG2a 211591 Anti-Pan-MAGUK [K28/86R-2b] Pan-MAGUK Human Mouse IgG2b 211592 Anti-Pan-Na...IgG2a 227070 Pan-MAGUK (Homo sapiens) recombinant mouse monoclonal antibody. Pan-MAGUK Human Chimera: M...

...blue. Protein band intensities are measured using ImageJ software and a standard curve is generated. The...concentration is calculated from the standard curve. Using ImageJ software, the band intensities of both the antibody...

... new window) . MAGeCK Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK). This tool can...

...transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image from Fenno et al., 2014. INTRSECT INTRSECT (intronic...opens in a new window) Wick ZC, Tetzlaff MR, Krook-Magnuson E. 2019. A novel population of long-range inhibitory...

...gRNAs that bind fluorescent proteins can be used to image genomic loci in live cells. RNA targeting : Cas13...EH, Weissman JS, Qi LS, Huang B. 2013. Dynamic imaging of genomic loci in living human cells by an optimized...