We narrowed to 205 results for: aav
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TypeBlog PostPublishedJuly 7, 2022, 1:15 p.m....elements for deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing...
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Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....to control the CRISPR system activity. Using an AAV vector may help with size limitations. In addition... -
The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m....plasmids alone. (And we expect that resources like our AAV Data Hub will help scientists save even more!) If... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....off-target effects, yet small enough to be packaged in AAV. A dCasMini-mediated adenine base editor allows for... -
22 Hot Plasmid Technologies from 2014
TypeBlog PostPublishedJan. 6, 2015, 5:21 p.m....been deposited to Addgene, including lentiviral and AAV expression vectors for certain variants. Klapoetke... infeasible. Finally, an adeno-associated viral (AAV) system enables greater spatio-temporal control over... either the Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et... -
27 Hot Plasmids from 2016
TypeBlog PostPublishedDec. 22, 2016, 3:03 p.m.... 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA AAV 74302 ...plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression and plasmids...Magneto2.0 in the mammalian brain after delivering it via AAV injection, finding that it could increase the activity...lines by inserting OsTIR1 into the "safe harbor" AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....it easier to package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts...Science, 362(6416): 839-842 (2018). 10.1126/science.aav4294 Ozcan, Ahsen, Krajeski, Rohan, Ioannidi, Eleonara... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....lentivirus, Adenovirus, and Adeno-associated virus (AAV). If you are wondering which virus fits you experiments... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....plasmids were developed to replace helper viruses in AAV production systems. We’re just skimming the surface... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....effects of various terminators in the context of AAV transgene expression levels. Transcription Termination... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....targeting expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of recombinase...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern CH, ... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....AdVs have already a packaging capacity larger than AAV vectors. This capacity is large enough to carry the... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....acids) means they can be delivered in vivo using AAV vectors. Having a way to shut off CRISPR-Cas systems... -
PCR Applications for Copy Number Variation Assays
TypeBlog PostPublishedApril 26, 2022, 1:15 p.m....Resources on the Addgene blog Droplet Digital PCR for AAV QuantificationPolymerase Chain Reaction Overview ... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog PostPublishedAug. 11, 2016, 2:23 p.m....to their transduction has been shown to increase AAV transduction efficiency by approximately 15-fold ... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....Lee et al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent versions...for the first time (Figure 3). The team also used AAV9 to deliver ASAP5 to the cortex and imaged voltage... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....amplify! Examples of this are the GC-rich ITRs of AAV genomic DNA encoding plasmids, or promoters such ... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....Plasmids Read Our Lentiviral Vector Guide Read Our AAV Guide ... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....low-capacity vectors, such as adeno-associated viral (AAV) vectors. In 2017, Zetsche et al. developed a Cpf1...