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We narrowed to 181 results for: grna

Showing: 161 - 180 of 181 results

  1. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...cassette encoding the cas9 gene and single guide RNAs (sgRNAs) with appropriate flanking homology into a germline...don't use a DNA vector that encodes both Cas9 and sgRNA. What if I want to work with gene drives? Then do...organisms but not wild organisms. For example, an sgRNA-only drive will spread exclusively through populations...express Cas9 from an unlinked locus, while a Cas9+sgRNA drive targeting a synthetic sequence will only spread...restore the wild-type sequence (save for the residual sgRNAs and possibly cas9 gene). An immunizing reversal...

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...recombination methods). By using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ ...Using an inducible Cas9 expression and removing the sgRNA plasmid after identifying successful knockouts limits... KanR. This strain is then transformed with the sgRNA cassette on a plasmid expressing mCherry and resistance... of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying Cas9 to...to allow the introduction of additional sgRNAs. Image reused from Neo et al., 2024 under CC-BY license...

  3. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...Veening is now available with one sgRNA per operon or gene. The sgRNA pool can also target core operons... cell lines. The Turner Lab Human messenger-RBP sgRNA Library targets human mRNA binding proteins with...operons in other pneumococcal strains. The BARBEKO sgRNA library uses CRISPR cytosine base editors to disrupt...

  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...indicating loci where SNPs reside on sgRNA target sequences for sgRNAs that target ancestry-associated genes...that could analyze CRISPR sgRNAs and report the likelihood of any given sgRNA having an ancestral bias....

  5. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...activity coupled with a customizable single guide RNA (sgRNA) that bears complementarity to a 20bp target sequence...multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds that ...modules are comparatively small allowing multiple sgRNA constructs to be introduced simultaneously in a ...

  6. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in human cell...limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better with Cas12, which...Workflow to assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation...

  7. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...including the cell line/tissue type, enzyme system, sgRNA library and other details relevant to screen result...gene-level scores provided by authors rather than sgRNA-level sequence read scores. The original supplemental...

  9. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...shRNA sequence specific to your gene’s mRNA or a sgRNA targeting an exon in your gene of interest, for ...with many applications. These libraries include sgRNAs targeting the entire genome, overexpression of ...

  12. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...those generated by multiplexed delivery of multiple sgRNAs. Since multiplexing is commonly used to create ...helps the researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type...

  13. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ... day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency. On the third...

  19. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...of PE protein components (Figure 1), an altered epegRNA delivered with an enhanced pU6cm promoter, viral...
Showing: 161 - 180 of 181 results