...conducted in the Hodaka Fujii Laboratory, please also see our interview with Dr. Fujii. References Campbell... The Fujii lab demonstrated this in mammalian cells by expressing dCas9 with an N-terminal 3xFLAG tag ...inactive form of Cas9 known as dCas9 (Jinek et al., 2012). Like Cas9, dCas9 can bind to a specific DNA sequence...targeting gRNA. But dCas9 does not cleave the DNA. Much of the research using dCas9 has focused on transcriptional...purification of a specific genomic region In 2013, the Hodaka Fujii Laboratory first described a method to purify...consisting of dCas9 and targeting gRNA, to identify molecular interactions at this site (Fujita and Fujii, 2013...molecule (Hoshino and Fujii, 2009). Other forms of enChIP, that do not use dCas9, require Zinc-finger ...