We narrowed to 28 results for: MYC

...commonly used Myc-tag. Who first started using it in protein tagging experiments? Why Myc? When did the...probe the functions of the human c-Myc gene (Evan, et al. 1985). Myc was one of the earliest identified...when Evan’s team generated their Myc antibodies. At first, anti-c-Myc [9E10] was just one of several new...   Myc1-9E10 and the Myc-tag today Since Munro and Pelham introduced the Myc tag to the world, it has...  Find recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...the commonly used anti-c-Myc [9E10] antibody come into play? We’ll dive into those questions in this post...post as we explore… the life and times of the myc tag.  Origins of protein tags For about as long as the...

...proto-oncogene c-Myc. Since the generation of the first anti-c-Myc [9E10] (Evan, et al., 1985), the Myc tag has...light-gated potassium channelrhodopsins Myc-tag antibody anti-c-Myc [9E10] now available! Improved voltage...doi.org/10.1101/2021.09.17.460684.)   Myc-tag antibody anti-c-Myc [9E10] now available! by: Ashley Waldron...to guess that the Myc-tag is one of them (and not just because of the title!) The Myc-tag is a string of... for ‘Myc tag’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10... of Addgene’s western blot for the myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the... the Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody here...

...monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 amino acid peptide (EQKLISEEDL) derived...negatively charged and 1 positively charged amino acids. Myc tags are frequently used for western blot, flow cytometry... However, protein purification is not ideal with Myc, as low pH conditions are required during the purification...affect protein function. Fun fact: The antibody to Myc was developed in 1985 and is still the most prevalent...Addgene has you covered with monoclonal antibodies to Myc for all of these species. HA The HA tag is a 9 amino...availability Plasmids 101: Protein Tags Antibodies: Of Myc and Men    ...

...either with a labeled anti- HA or -MYC antibody or with an anti-HA or -MYC primary and a labeled secondary... Spaghetti Monster: HA (H) or Myc (M) versions Labeled anti-HA or -Myc antibody Staining cultured cells... are highly antigenic tags that have 10 HA or 10 MYC tags built into a GFP scaffold. Some GFP nanobodies...RANbody detection with a directly labeled anti-HA or -MYC antibody is less sensitive than HRP RANbody detection...sections When detected with a labeled anti-HA or -MYC antibody, approximately as sensitive as detecting...secondary antibody staining HA Tagged GFP, RFP, H2A2B Myc Tagged RFP, H2A2B Chicken IgY (Y) Labeled Anti-Chicken...

...enhances iPSC generation as does replacing C-MYC with L-MYC [10, 11]. While the mechanisms underlying the...addition of an shRNA for P53 and replacing C-MYC with L-MYC in the episomal reprogramming cocktail by Okita...reprogramming factors- OCT3/4, SOX2, KLF4, and C-MYC [1]. For the derivation of clinical grade hiPSCs,...that encoded six factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method...pCXLE-hSK 27078 SOX2 and KLF4 pCXLE-hUL 27080 L-MYC and LIN28 pCXLE-hOCT3/4-shp53-F 27077 OCT3/4 and...direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A, 2010. 107(32): p. 14152...

...resistance.  The MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data...several MYC-dependent cancer cell lines. It can be applied for identification of essential MYC binding...

...epitope tags? Short epitope tags like FLAG, HA, and Myc can adopt a number of different conformations and... fusions to inntags or traditional FLAG, HA, and Myc tags. Impressively, IT6-GFP did not reduce yeast ...

...protocol starts out with anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the...the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag to the end of each sybody candidate...

...fraction of the price; you can choose 10xHis-, MBP-, or myc-tagged SerMNucA, depending on your preference or ...SerMNucA (Benzonase/Turbonuclease) as 10xHis, MBP or myc-tag fusions. protocols.io, doi: https://dx.doi.org...

...to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses of the affinity tag...step purification with amylose, but wicked huge Myc EQKLISEEDL  1.2 Affinity Frequently used for western...

...fluorescent proteins (GFP and YFP) and protein tags (c-MYC and HA). We have also created the Monoclonal Antibody...

... viral particles that express oncogenes, such as Myc. Viral vectors also have the largest genomic footprint...transgenes is a concern as well, and reactivation of Myc or other oncogenes after differentiation has been...including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids if you’re interested...

...different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry...

...and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include proteins like nonstructural...

...four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic cell to revert...additional pluripotency stage) Less Safety Less (C-MYC, a known oncogene is one of the reprogramming factors...

... transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming somatic...article have been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation...

...Figure 1: A) Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B...

...of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated from mouse fibroblasts...

... T2A-TurboGFP-PEST), and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann...