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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6 results for: Sleeping Beauty

Showing: 1 - 6 of 6 results

  1. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...interest insertion site.   The Sleeping Beauty transposon system The Sleeping Beauty (SB) transposon system is...that can translocate within the genome. The Sleeping Beauty transposase gains its name from the fact that...changes. The basic functional version of the Sleeping Beauty transposon (SB10), developed in 1997, continues...efficiencies of integrating viral vectors. New Sleeping Beauty transposase vectors Even more recently, Eric...constitutively or regulated by doxycycline. The Sleeping Beauty transposase is proving an exciting tool for...presented results from a clinical trial using the Sleeping Beauty transposase as a nonviral vector for CD19-directed...  References 1. Kowarz E, et al. Optimized Sleeping Beauty transposons rapidly generate stable transgenic...

  2. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...research tools: Sleeping beauty, PiggyBac, and Tol2.  Sleeping beauty Sleeping Beauty is a synthetic transposable...in fish (Sandoval-Villegas et al., 2021). Sleeping Beauty’s preferred target site for integration is TA...integration efficiency decreases with cargo size. Sleeping Beauty has a close-to-random integration profile in...transposition activity (Voigt et al., 2016). Sleeping Beauty plasmids  piggyBac Although its name suggests...islands, and DNaseI hypersensitivity sites. Like Sleeping Beauty, piggyBac has an integration efficiency in ...caused albinism (Koga et al., 1996). Unlike Sleeping Beauty and piggyBac, Tol2 has a weak consensus sequence...integration in human cells than piggyBac and Sleeping Beauty. Minimal Tol2 or miniTol2 is a truncated version...

  3. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...himself.   Sleeping Beauty, a transposon system from ancient fish Surprisingly, Sleeping Beauty is not only...awakened from a long evolutionary sleep,” it was aptly named Sleeping Beauty (Ivics et al., 1997). We have... in rooms such as Groucho, Sonic Hedgehog, Sleeping Beauty, Cookie Monster, Spaghetti Squash, Tinman, ...wheel but also a biological transposon system. Sleeping Beauty is a synthetic transposon system that can be...this transposon system? Scientists can use Sleeping Beauty transposons as a non-viral system to integrate...
Showing: 1 - 6 of 6 results