Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
Type
Blog Post
Published
Feb. 24, 2015, 7:50 p.m.
...hits.”
Two-way CRISPR screening
In October, Jonathan Weissman’s lab introduced additional pooled CRISPR libraries...transcription start site in those 15,977 human genes.
Weissman and his colleagues demonstrated in a paper in ...genome for gene control over a 1,000-fold range.
Weissman explained that there are two levels of control...of genes that can be manipulated at one time. Weissman’s group has made standard protocols available and...Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman JS. Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016...