Fluorescent Proteins 101: Luciferases
Type
Blog Post
Published
July 22, 2025, 1:15 p.m.
...Well-established
Natural and engineered luciferases
Although firefly luciferase was the first bioluminescent reporter...ATP
No
Luciferase reporter assays for monitoring gene regulation
Since firefly luciferase was first....
Figure 1: Firefly, from Wikimedia Commons.
The North American firefly, Photinus pyralis, ...have engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...Oplophorus gracilirostris) luciferase and engineered to be extra bright. Other luciferases have been modified ...mammalian systems. Our luciferase collection page has a detailed list of luciferase options.
Table 2: Properties...element is cloned upstream of the luciferase gene, and expression of luciferase is measured via the light produced...