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We narrowed to 5 results for: firefly luciferase

Showing: 1 - 5 of 5 results

  1. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...expressing luciferase Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was ...Well-established Natural and engineered luciferases Although firefly luciferase was the first bioluminescent reporter.... Figure 1: Firefly, from Wikimedia Commons. The North American firefly, Photinus pyralis, ...have engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...Oplophorus gracilirostris) luciferase and engineered to be extra bright. Other luciferases have been modified ...mammalian systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties...element is cloned upstream of the luciferase gene, and expression of luciferase is measured via the light produced...

  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...Protein, codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from ...than our more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not... creation of NanoLuc® Luciferase from a rather dull 19kDa Oplophorus luciferase by Promega’s Advanced ...Direct injection of plasmids for Antares or firefly luciferase into mouse livers followed by i.v. injection...coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce...isn’t science better when we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies...bioluminescence to overcome these issues as traditional luciferases emit at near optimal wavelengths (Fluc emits ...

  3. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ... of viral infection), producing firefly luciferase. Firefly luciferase can readily be measured to quantify... a single (non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene...template to transcribe the reporter gene (e.g. firefly luciferase). The polymerase recognizes the gene start...

  4. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use in ...alternative to the widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids... issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash...insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation of its substrate, luciferin...containing Cas9, EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with...gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral vector containing...et al. Nat Methods. 2011. High-throughput luciferase reporter system for detection of pre-mRNA splicing...

  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Protein A-Renilla luciferase and the “prey” proteins are tagged with Firefly luciferase. The bait and prey...normalized to assess interactions. Higher relative firefly luciferase activity in the Co-IP compared of a control...immunoprecipitated from the cells using the Protein A tag. The luciferase activities can then be measured and normalized...
Showing: 1 - 5 of 5 results