We narrowed to 6 results for: firefly luciferase

...For most people, the most familiar source of bioluminescence is the firefly. Along with charming nighttime.... Figure 1: Firefly, from Wikimedia Commons. The North American firefly, Photinus pyralis, ...Well-established Natural and engineered luciferases Although firefly luciferase was the first bioluminescent reporter...have engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...Oplophorus gracilirostris) luciferase and engineered to be extra bright. Other luciferases have been modified ...mammalian systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties...ATP No Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was first...

...than our more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not...Protein, codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from ... creation of NanoLuc® Luciferase from a rather dull 19kDa Oplophorus luciferase by Promega’s Advanced ...coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce...This post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since...isn’t science better when we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies...bioluminescence to overcome these issues as traditional luciferases emit at near optimal wavelengths (Fluc emits ...

... of viral infection), producing firefly luciferase. Firefly luciferase can readily be measured to quantify... a single (non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene...template to transcribe the reporter gene (e.g. firefly luciferase). The polymerase recognizes the gene start...

...Protein A-Renilla luciferase and the “prey” proteins are tagged with Firefly luciferase. The bait and prey...immunoprecipitated from the cells using the Protein A tag. The luciferase activities can then be measured and normalized...normalized to assess interactions. Higher relative firefly luciferase activity in the Co-IP compared of a control...

...Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use in ...alternative to the widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids... issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash...insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation of its substrate, luciferin...containing Cas9, EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with...gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral vector containing...et al. Nat Methods. 2011. High-throughput luciferase reporter system for detection of pre-mRNA splicing...

...Renilla luciferase variant fused to an FP; when supplied with a luciferase substrate, the luciferase transfers...Fluorescent imaging techniques have become indispensable tools for molecular and cell biologists over the...problems of low luminescence By itself, Renilla luciferase has a low quantum yield, and when transiently...Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent technology...transfer (BRET). Photons emitted by a Renilla luciferase variant are used to excite the fused fluorescent...need for an excitation light source. Instead, a luciferase substrate like coelenterazine is supplied in ...eNL constructs use the brightest characterized luciferase, NanoLuc, and its substrate furimazine. In addition...