We narrowed to 24 results for: gibson assembly

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Sept. 6, 2017, 1:01 p.m.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Sept. 26, 2024, 1:15 p.m.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Sept. 10, 2020, 11:45 a.m.

REPLACR-mutagenesis is a new in vivo method for mutating your plasmid constructs. Learn how REPLACR stacks up against other mutagenesis techniques.

Feb. 10, 2016, 3:30 p.m.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

Feb. 22, 2017, 3:30 p.m.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Aug. 21, 2018, 12:31 p.m.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

Feb. 7, 2017, 3:30 p.m.

A list of free online molecular biology tools for plasmid mapping, DNA and protein sequence analysis, primer design and more.

Sept. 12, 2023, 1:15 p.m.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Aug. 27, 2015, 2:30 p.m.

Using plasmids for transformation, cloning, or transfection? Different uses mean you'll need different purities of DNA for your applications.

June 16, 2020, 1:25 p.m.

In this troubleshooting guide, find step-by-step tips and tricks for troubleshooting your cloning experiment.

Sept. 24, 2019, 12:55 p.m.

PCR can be used to append the appropriate restriction sites to your insert and facilitate downstream cloning. Read on for tips on this process.

March 29, 2016, 2:30 p.m.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

July 7, 2015, 3:36 p.m.

How much time does it take to clone a plasmid and how much does it cost? We do some back of the envelope math to try and figure it out!

March 23, 2023, 1:15 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Dec. 22, 2016, 3:03 p.m.

Use CcdB to enhance the efficiency of your next plasmid cloning experiment.

Dec. 8, 2016, 3:30 p.m.

Colony PCR is a useful technique for quickly screening for successful constructs after a plasmid cloning experiment.

May 12, 2016, 2:30 p.m.

Learn what a high fidelity DNA ligase is and when you might need one.

March 6, 2018, 1:49 p.m.

Learn how to use recombinase-based state machines from the Lu lab to monitor cellular events and regulate gene expression.

July 28, 2016, 2:30 p.m.