We narrowed to 8 results for: mKO
-
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m....previously developed monomeric fluorescent proteins mKO and mCherry, respectively. While Tsuboi et al developed...somewhat serendipitously while attempting to enhance mKO in other ways, Subach et al made a concerted effort...
-
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....tendency, our choice would be mKOk-2A-mTq2; it is much brighter than mKO2-2A-mTq2 according to this data... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....can be used in the OSER assay including CytERM-mKOkappa, CytERM-mTurquoise2, and CytERM-dTomato. When...structure. Based on these data, we conclude that mKOk has dimerization tendency and is not suitable for... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....Transfer Vectors Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...with GFP expression. Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is... -
To Codon Optimize or Not: That is the Question
TypeBlog PostPublishedNov. 12, 2020, 2:15 p.m....ability to selectively silence neurons in vivo (Slimko and Lester, 2003). Addgene has a large selection...1281–1295 . https://doi.org/10.1093/nar/15.3.1281 Slimko EM, Lester HA (2003) Codon optimization of Caenorhabditis... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....AAV transfer plasmid! References: Naso MF, Tomkowicz B, Perry WL III, Strohl WR (2017) Adeno-Associated... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....Leipzig: S. Hierzel. J.A Dent, A.G. Polson, M.W. Klymkowsky. (1989) Development, 105.61-74. Pubmed. J. Sharpe... -
15 Hot Plasmids from 2017
TypeBlog PostPublishedJan. 9, 2018, 3:02 p.m....for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that have standard cloning sites...