...project from the Neuroscience Information Framework (NIF) called the Resource Identification Initiative. During...journals and their scientific communities aim to significantly improve the reproducibility and reliability ...

...determines if there is statistical significance among data sets. Significance is a numerical value which quantifies... they deserve that oh-so coveted asterisk of significance? You’ve already done the heavy lifting, so don...assess if the mean of an experimental group is significantly greater or lesser than the mean of a control...variables. This test will tell you if there are significant differences between the means of your groups....on a five-sample data set yields statistical significance, you won’t know if only two of those samples...pairs within the five is different. To identify significant pairings, additional tests, referred to as post-hoc...statistical test   Post hoc tests Knowing that significance exists somewhere amongst multiple groups is ...

...research vision is your approach to resolving a significant knowledge gap in your field or even creating ...field, and interesting. For example, “there is a significant need for healthy livers for transplants. Growth...knowledge gap you plan to address and why is it significant? What is the problem you will study? Why this... down your problem and then explain why it’s significant and innovative. For example, "while it is possible... to your research problem. Creating a coherent unified story and model can help make your ideas easier...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...

...interactions for subsequent sheering of DNA by sonification. Though it helps capture weaker or more transient...Washington. CUT&RUN doesn’t require fixation and significantly decreases background noise, reducing the read...dissections. With CUT&RUN, we have had success with significantly fewer wings, such that enough tissue for an ...improvements to the initial CUT&RUN protocol, the most significant being the usage of a strong detergent to permeabilize...extraction. They report that this change offers a significant improvement and simplification of the original...

...dissolved at a higher concentration, and results in uniform NB biotinylation. Once induced, the NBs could be...CRISPRa system for human pluripotent stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene...dCas9 and the gRNA. This expression can vary significantly depending on transgene design and delivery. ...developed fluorescently labeled piggyBac vectors for uniform and sustained expression of single or multiplexed....1038/s41598-020-57500-1 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...

... Proteins For Molecular Biology Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent.... 7. 25 Work From Home Tips for Scientists by Jennifer Tsang These tips were written early on in the...Five DNA Quantification Methods to Consider by Jennifer Tsang The nanodrop vs. spec debate dominates ... Jennifer Tsang...

... and Negative Selection for Plasmid Cloning by Jennifer Tsang There are many strategies for finding your...Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi...Precision and Flexibility to CRISPR Editing by Jennifer Tsang Prime editing is a new CRISPR technique... Jennifer Tsang...

...portion of optogenetics (though it does require a significant amount of equipment, most of which is relatively...point; the fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency...stimulation, the rate of movement of the animal significantly increases. Before you get your experiment started...demands of using these wireless devices are significantly higher than standard “wired” optogenetics but...

...were less significant than when non-targeting guides are used as controls. Less significant p-values lead...guides and may more accurately determine the significance of hits in non-growth screens. Off-target cutting...of on-target activity. However, there was no significant difference in depletion of ricin sensitizer genes...

...Addgene, I was about to have a check-in with my boss Jennifer. I had finally found my footing with the company... for what we thought would be a couple months. Jennifer and I spent about an hour that morning scrambling...know cameras, but we needed someone else. Design: Jennifer thankfully volunteered, and we decided to focus...action would be to use an iPad to shoot.  Build: Jennifer created a mobile camera area that included iPad...

...libraries also include 2,400 gRNAs without any significant hit across the E. coli genome as a negative control... fold and >3,800 fold in human cells with no significant reduction in editing efficiency. Read the paper... Jennifer Tsang...

... of twofold or greater with good statistical significance (Taylor, 2017). With the right conditions and...multiplexing is quite difficult and requires significant optimization to ensure the results are correct...multiplexing your reference gene set can save significant plate space and may be worth the initial effort...statistical analysis to determine if there are any significant differences between your experimental values ...

...annotate genetic perturbations that may play significant roles in cancer pathogenesis. Their work culminated...mutations were found to be “neutral,” having no significant effect on cell viability compared to a wild-type...Approximately ~22% of the mutations, however, significantly increased cell viability, and these “activators...

...an attempt to present only the most novel or significant findings, and combined with powerful cognitive...QRPs) can quickly lead us to find statistically significant results that are not robust. Sampling noise There...are too easy to overlook when presented with a significant result that may be the result of noise, p-hacking...

...enzymes outlined below as well. Cas12a also has significant therapeutic promise due to the fact that it is...target sequence Similar to Cas12, it is also significantly smaller in size than traditional Cas9. Another... not display collateral RNA cleavage and is significantly less toxic to mammalian cells than Cas13 (likely...

...noncovalent dimers. This property of EGFP can lead to significant artifacts. If you're using green fluorescent ... Fusion of an FP to a transmembrane protein significantly increases the effective concentration of the...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...

...allows you to physically magnify a sample so you don’t have to optically magnify quite so much. Created ...Klimas A, Gallagher BR, Wijesekara P, et al (2023) Magnify is a universal molecular anchoring strategy for...

...caudal NST. (A-C) Low-magnification images showing viral spread. (D-F)  High-magnification images immuno-stained...minimum n = 2, and well labeled high- and low-magnification images demonstrating vector performance) will...

...you! Many thanks to our Guest Blogger, Jennifer Rivers! Jennifer Rivers ScM, MLS(ASCP)CM is a research ...This post was contributed by guest blogger, Jennifer Rivers, a research microbiologist specializing in...

...enzyme can lead to off targets and result in significant, negative effects in RNA targeting experiments...non-specific activity. Its major advantage is the significantly reduced amount of off-target toxicity compared...using Prime editing alongside some creative (and significant!) protein engineering. They combined Prime editing...