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Showing: 1 - 2 of 2 results
  1. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion ...175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR for activation...pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene #110821 ) VPR ( Addgene #63798) Plasmids from...
  2. CRISPR Guide

    Type
    Collection
    ...activation domains in series (e.g. dCas9-VPR, panel C ); or co-expression of dCas9-VP64 with a modified scaffold...One method uses orthogonal dCas9’s (e.g., S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent...permanently modifying the genome dCas9-repressor (such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements... a nuclease dead Cas9 (dCas9) molecule that cannot cleave target DNA. The dCas9 molecule retains the ability...target genes. The simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single...-tagged dCas9 and antibody-activator effector proteins (e.g. SunTag system , panel B ); dCas9 fused to...catalytically inactive dCas9 fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...
Showing: 1 - 2 of 2 results