Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 4 results for: Free Use GFP

Showing: 1 - 4 of 4 results

  1. Adenovirus Guide

    Type
    Guide
    ...limited their use in therapeutic applications. However, this has also made them the most widely used viral vector...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...viral vector production. During experiments, GFP can be used to sort cells infected with rAdV vectors, or...based on the use of pAdEasy-1. Created with BioRender.com. AdMax™ AdMax™ is another well used rAdV vector...disadvantage for the use of rAdV vectors in gene therapy but has, however, proven to be very useful for designing...that contain IRES-GFP. Pseudotyping The production of viral vectors (or viruses) using viral envelope proteins...that do not contain GFP. Recombinant adenoviral vector (rAdV) Modified form of AdV used to deliver genetic...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Production Using Packaging Cell Lines Gamma-retroviral vectors can be packaged using helper-free cell lines...Common Uses of Gamma-Retroviral Vectors Due to their integration, gamma-retroviral vectors are useful research...-activated cell sorting (FACS) is used to sort cells expressing GFP and later, sorted cells are expanded...clinical studies and commercial purposes. The use of helper-free production cell lines also saves time in ...viral vectors (or viruses) using viral envelope proteins from different species. Used to alter infectivity...variety of cell types than retroviruses. Both lentiviruses and gamma-retroviruses use the same packaging genes...Pseudotyping Common Uses Safety Resources Plasmid Elements Glossary Gamma-retroviruses are a subtype of ...

  3. Modular Cloning Guide

    Type
    Guide
    ...circuits using Golden Gate-based cloning methods for use in mammalian cells. These can be used with plasmids...nickase, dCas9, or dCas9-FokI fusion for use in human or mouse systems. Fragmid Genome Engineering, CRISPR...resulting in a 4-bp fusion site in between. MoClo can be used for a variety of applications—like building synthetic...cloning vectors (Level 0, 1, and 2) which can be used in successive assembly steps. Before beginning, ...standardized and characterized parts that can be used for bottom-up hierarchical assembly of single and...Yeast Expression Paul Young 34 plasmids that can be used in conjunction with the MoClo Yeast Toolkit (YTK...integration in fission yeast S. pombe . These can be used with plasmids from the MoClo-YTK . MoClo Pichia ...

  4. CRISPR Guide

    Type
    Guide
    ...these PAM-flexible Cas9s are useful for precision edits, they can be used for virtually any CRISPR application...concentration too low to be used in experiments and must be amplified before use (Figure 8C), following the...libraries have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR libraries from...co-opted for use in eukaryotic cells . The Cascade complex is recruited to target DNA using a gRNA and ...bacterial genomic region used in pathogen defense CRISPRa CRISPR Activation; using a dCas9 or dCas9-activator...multiple genes at once; using dual nickases to generate a knockout or gene edit; or using Cas9 to generate large...will look for relevant hits using NGS technology. For more detail on using CRISPR libraries for screens...
Showing: 1 - 4 of 4 results