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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6 results for: mPA-GFP

Showing: 1 - 6 of 6 results

  1. Adenovirus Guide

    Type
    Guide
    ...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...throughout viral vector production. During experiments, GFP can be used to sort cells infected with rAdV vectors...recombinant adenoviral vector production that contain IRES-GFP. Pseudotyping The production of viral vectors (or...adenoviral vector production that do not contain GFP. Recombinant adenoviral vector (rAdV) Modified form...have limited restriction sites or may only be compatible with certain cloning methods (such as a Gateway... so be sure to confirm your chosen plasmid is compatible with cloning methods available in your lab. When... package larger cargo. CRISPR-Cas9 Delivery As compared to other viral vectors, rAdV vectors can provide...

  2. Plan Your Experiment

    Type
    Guide
    ...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...repair template and displays reduced efficiency compared to NHEJ knockout. Base editors can make a limited...our AAV Guide ) ∼4.5 kb packaging limit (only compatible with smaller Cas enzymes) Transient or stable...Cas9 and gRNA Choose a delivery method that is compatible with your experimental system. CRISPR efficiency...

  3. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead of ... sorting (FACS) is used to sort cells expressing GFP and later, sorted cells are expanded into a cell ...have limited restriction sites or may only be compatible with certain cloning methods (such as a Gateway... so be sure to confirm your chosen plasmid is compatible with cloning methods available in your lab. When...

  4. Lentiviral Vector Guide

    Type
    Guide
    ...selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead of ... sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More information...third-generation packaging plasmids. For a summarized comparison of second- and third-generation systems, please...have limited restriction sites or may only be compatible with certain cloning methods (such as a Gateway... so be sure to confirm your chosen plasmid is compatible with cloning methods available in your lab. When...lentiviral preference for transcribed genes. Studies comparing the lentivirus HIV and the gamma-retrovirus MMLV...

  5. Optogenetics Guide

    Type
    Guide
    ...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...H134 Widely used variant with larger photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster...applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nat Methods. 9(...

  6. CRISPR Guide

    Type
    Guide
    ...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...nuclease pair for every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became...it meets two conditions: The sequence is unique compared to the rest of the genome The target is present...throughout the genome, called off-targets, that can impact your experiment. There are many online tools available...effects Sniper-Cas9 - less off-target activity; compatible with truncated gRNAs to increase specificity ... offer high efficiency and low indel formation compared to HDR. Prime editing requires more optimization...) includes tnsA , tnsB , tnsC , and tniQ . In comparison, type V-K includes just tnsB , tnsC , and tniQ...
Showing: 1 - 6 of 6 results