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Showing: 1 - 4 of 4 results
  1. Adenovirus Guide

    Type
    Guide
    ...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...vectors that contain IRES-GFP pShuttle Class of transfer vectors that do not contain GFP Additional Vocabulary...throughout virus production. During experiments, GFP can be used to sort cells infected with adenovirus...pAdEasy-1 packaging plasmid 911E4 cells Cell line compatible with the pAdEasy-2 packaging plasmid; Supplies...
  2. Lentiviral Guide

    Type
    Guide
    ...another marker, such as GFP. A researcher can use FACS to sort cells expressing GFP and later expand these...restriction sites but compatible with the Gateway® cloning system? Gateway® compatible vectors use recombination...generation or 3rd generation packaging system. For a comparison of the key differences between the 2nd and 3rd...lentiviral preference for transcribed genes. Studies comparing the lentivirus HIV and the retrovirus MMLV indicate...using this site include subcloning or appending compatible restriction sites onto the insert of interest...until the gene of interest is flanked by sites compatible with those in the vector into which one ultimately...
  3. CRISPR Guide

    Type
    Guide
    ...marker like GFP, researchers have turned dCas9 into a customizable DNA labeler compatible with fluorescence...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence...nuclease pair for every genomic target. Due to its comparative simplicity and adaptability, CRISPR has rapidly...it meets two conditions: The sequence is unique compared to the rest of the genome. The target is present...gRNA targeting sequence is for the genomic target compared to the rest of the genome. Ideally, a gRNA targeting...
  4. Optogenetics Guide

    Type
    Guide
    ...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...H134 Widely used variant with larger photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster...applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nat Methods. 9(...
Showing: 1 - 4 of 4 results