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Showing: 1 - 5 of 5 results
  1. Adenovirus Guide

    Type
    Guide
    ...The pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene...pShuttle Class of transfer vectors that do not contain GFP...During experiments, GFP can be used to sort cells infected with adenovirus, or to verify that the infection...adenoviral vector pAdTrack Class of transfer vectors that contain IRES-GFP...containing the pAdEasy-1 packaging plasmid 911E4 cells Cell line compatible...
  2. Chemogenetics Guide

    Type
    Guide
    ...Serotype-dependent spreading profiles of AAV-mediated GFP expression in the marmoset cerebral cortex...spread using CMV, CaMKII, and SynI promoter-driven GFP expression *significantly different from AAV1...5 1.3 ± 0.3 8 No statistical difference observed between AAV1, 5, 8, 9 9 1.2 ± 0.4 1Mean of GFP...The serotype of the AAV used for in vivo transduction can impact and enhance the delivery of constructs...Comparative analyses of adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and...
  3. Lentiviral Guide

    Type
    Guide
    ...A researcher can use FACS to sort cells expressing GFP and later expand these cells into a cell line....have selectable markers conferring resistance to an antibiotic, but do encode another marker, such as GFP...For a comparison of the key differences between the 2nd and 3rd generation packaging systems, see the...Studies comparing the lentivirus HIV and the retrovirus MMLV indicate that the viral integrase plays...How do I clone my insert into a transfer vector with no viable restriction sites but compatible with...Gateway® compatible vectors use recombination in order to generate clones containing the insert of interest...basically shuffling restriction sites between vectors until the gene of interest is flanked by sites compatible...
  4. CRISPR Guide

    Type
    Guide
    ...GFP) or selection marker to identify and enrich positive cells Packaging and envelope plasmids provide...Genomic Loci Using Fluorophores Using catalytically inactive dCas9 fused to a fluorescent marker like GFP...GFP) to identify and enrich positive cells, or selection marker to generate stable cell lines (for details...PMID: 29891532 CRISPR-Based Technologies: Impact of RNA-Targeting Systems. 2018. Terns MP....Due to its comparative simplicity and adaptability, CRISPR has rapidly become the most popular genome...PMID: 30166441 Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.. 2018....Compared to techniques like fluorescence in situ hybridization (FISH), CRISPR imaging offers a unique...
  5. Optogenetics Guide

    Type
    Guide
    ...light-inducible dimerization tags (TULIPs), plasmids allow cloning of protein coding sequences with GFP-LOVpep...Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins...from Chlamydomonas reinhardtii (CrChR2) 470 ChR2/H134 Widely used variant with larger photocurrent compared...
Showing: 1 - 5 of 5 results