Protocol - pLKO.1 – TRC Cloning Vector
Type
Protocol
...Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral particles....Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells GenHunter: #Q401 ... plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-free OPTI-MEM TIP... any nucleotide, R is a purine (A,G), and Y is a pyrimidine (C,U). G-C content should be 36-52%. Sense....3 Protocol for lentiviral infection and selection G. Safety H. References H.1 Published articles H.2 Web.... Incubate for 20-30 minutes at room temperature. g. Retrieve HEK-293T cells from incubator. The cells...positive control for the puromycin selection. Days 4+: g. Change to fresh puromycin-containing media as needed...