HMBP-3C-STAR.66-285
(Plasmid #100094)

• Purpose: expresses MBP-tagged STARD1 protein domain (residues 66-285)
• Depositing Lab: Nikolai Sluchanko
• Publication: Sluchanko et al Protein Expr Purif. 2016 Mar;119:27-35. doi: 10.1016/j.pep.2015.11.002. Epub 2015 Nov 7.

Backbone

• Vector backbone: HMBP-3C
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STAR protein
• Alt name: STARD1
• Alt name: steroidogenic acute regulatory protein
• Species: H. sapiens (human)
• Insert Size (bp): 670
• Entrez Gene: STAR (a.k.a. STARD1)
• Promoter: Ptac
• Tag / Fusion Protein:
  • His-tagged MBP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: AACAACCTCGGGATCGAG

Resource Information

• Supplemental Documents:
  • STAR.66-285_insert.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The STARD1 domain sequence was subcloned after 3C site to obtain 3C cleavable His6-MBP-3C-STAR construction.