Purpose(Empty Backbone) Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||119749||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5000
Modifications to backboneRfB Gateway cassette cloned into pGEX6P1 SmaI site
Vector typeBacterial Expression
- Promoter tac promoter
/ Fusion Protein
- GST (N terminal on insert)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsIdeally grow with Ampicillin and Chloramphenicol in growth medium
Copy numberHigh Copy
- Cloning method Gateway Cloning
- 5′ sequencing primer PGEX5' - GGGCTGGCAAGCCACGTTTGGTG
- 3′ sequencing primer PGEX3' - CCGGGAGCTGCATGTGTCAGAGG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX6P1-DEST was a gift from Andrew Jackson & Martin Reijns (Addgene plasmid # 119749 ; http://n2t.net/addgene:119749 ; RRID:Addgene_119749)