Addgene - pDest-527 Plasmid Data

Plasmid 11518: pDest-527

Gene/insert name:
None
Fusion protein or tag:
His6
Terminal:
N terminal on backbone
Vector backbone:
pDest-560
(Search Vector Database)
Backbone manufacturer:
PEL
Vector type:
Bacterial Expression ; Gateway Destination Vector
Backbone size (bp):
8266
Cloning method:
Gateway Cloning
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin and Chloramphenicol
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
Must be grown in a strain resistant to ccdB (DB3.1, ccdB Survival, or F').
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
Dominic Esposito
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11518" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

11086: pDEST-periHisMBP