pDest-565
(Plasmid #11520)

• Purpose: (Empty Backbone) Gateway destination vector for bacterial expression; His,GST tag
• Depositing Lab: Dominic Esposito
• Publication: Gateway Destination vectors for E. coli expression (unpublished)

Backbone

• Vector backbone: pDest-560
• Backbone manufacturer: PEL
• Backbone size (bp): 8932
• Vector type: Bacterial Expression ; Gateway Destination Vector
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • GST (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: Requires growth in a strain resistant to ccdB (DB3.1, ccdB Survival, or F')
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GST gene from Promega pGEX vector
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry