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Purpose(Empty Backbone) Gateway destination vector for bacterial expression; His,GST tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 11520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDest-560
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Backbone manufacturerPEL
- Backbone size (bp) 8932
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Vector typeBacterial Expression ; Gateway Destination Vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Growth instructionsRequires growth in a strain resistant to ccdB (DB3.1, ccdB Survival, or F')
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- His6-GST (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGST gene from Promega pGEX vector
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDest-565 was a gift from Dominic Esposito (Addgene plasmid # 11520 ; http://n2t.net/addgene:11520 ; RRID:Addgene_11520)