pDest-565
(Plasmid #11520)

• Purpose: (Empty Backbone) Gateway destination vector for bacterial expression; His,GST tag
• Depositing Lab: Dominic Esposito
• Publication: Gateway Destination vectors for E. coli expression (unpublished)
• Sequence Information:

  • Depositor Sequences: Full (1)
  • Addgene Sequences: Partial (4)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	11520	Plasmid sent as bacteria in agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pDest-560
  (Search Vector Database)
• Backbone manufacturer: PEL
• Backbone size (bp): 8932
• Vector type: Bacterial Expression ; Gateway Destination Vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: Requires growth in a strain resistant to ccdB (DB3.1, ccdB Survival, or F')
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • His6-GST (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GST gene from Promega pGEX vector
• Terms and Licenses:
  • UBMTA
• Articles Citing this Plasmid:
  • 6 References