Addgene - pDest-566 Plasmid Data

Gene/insert name:
None
Fusion protein or tag:
His6-MBP
Terminal:
N terminal on backbone
Vector backbone:
pDest-560
(Search Vector Database)
Backbone manufacturer:
PEL
Vector type:
Bacterial Expression
Vector type:
Gateway Destination Vector
Backbone size w/o insert:
9367
Cloning method:
Gateway Cloning
5' sequencing primer:
N/A List of Sequencing Primers
3' sequencing primer:
N/A
Bacterial resistance:
Ampicillin and Chloramphenicol
Growth strain:
DB3.1
Growth temperature (℃):
37
Growth instructions:
Must be grown in a strain resistant to ccdB (DB3.1, ccdB Survival, or F').
High or low copy:
Low Copy
Person or lab that originally
cloned the gene/insert:
MBP fragment from New England Biolabs pMAL-c2 vector
Sequence: View sequences (2)
Principal Investigator:
Dominic Esposito
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11517" in your Materials and Methods section.