Addgene - pDEST-periHisMBP Plasmid Data

Plasmid 11086: pDEST-periHisMBP

Gene/insert name:
His6/maltose binding protein
Alt name:
MalE
Alt name:
MBP
Insert size:
8357
Species:
E. coli
Fusion protein or tag:
His6
Terminal:
N terminal on backbone
Fusion protein or tag:
MBP
Terminal:
N terminal on backbone
Mutation:
His 6 added between N-terminal signal peptide and mature MBP
Vector backbone:
pMal-C2
(Search Vector Database)
Backbone manufacturer:
New England Biolabs
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
6000
5' sequencing primer:
tac promoter List of Sequencing Primers
Bacterial resistance(s)
Ampicillin and Chloramphenicol
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
plasmid must be grown in gyrA mutant resistant to the toxin CcdB
High or low copy:
Low Copy
Sequence: View sequences (3)
Principal Investigator:
David Waugh
Terms and Licenses:
MTA

Comments:

The MBP ORF can be sequenced using 5'-GAG CGG ATA ACA ATT TCA CAC AGG-3' as a tac promoter sequencing primer (in the forward direction). To sequence inserts fused to the C-terminus of MBP, the primers are 5'-GAT GAA GCC CTG AAA GAC GCG CAG-3' (forward) and 5'-GCA AGG CGA TTA AGT TGG GTA ACG C-3' (reverse).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli. Nallamsetty et al (Protein Sci. 2005 Dec . 14(12):2964-71. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11086" in your Materials and Methods section.

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