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Plasmid 8827: pRK793
  • TEV protease, S219V mutant

  • tobacco etch virus protease

  • 750

  • His

  • N terminal on insert

  • polyarginine

  • C terminal on insert

  • MBP (with TEV)

  • N terminal on insert

  • S219V mutation

  • pMal-C2
    (Search Vector Database)

  • New England Biolabs

  • Bacterial Expression

  • 6700

  • SacI

  • No

  • BamHI

  • No

  • N/A List of Sequencing Primers

  • M13-F20

  • Ampicillin and Chloramphenicol

  • BL21(DE3)-RIL

  • 37

  • Shifting the temperature from 37C to 30C during induction maximizes the yield of soluble TEV protease. Ampicillin for p793; Chloramphenicol for pRIL.

  • Low Copy

  • ATCC

  • View sequences (3)
  • David Waugh

  • UBMTA

Comments: 

Bacterial strain: E.coli BL21(DE3)-RIL (Stratagene).

pRK793 overproduces the catalytic domain of tobacco etch virus (TEV) protease in the form of an MBP fusion protein that cleaves itself in vivo to yield a TEV protease catalytic domain with an N-terminal His-tag and a C-terminal polyarginine tag.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Kapust et al (Protein Eng. 2001 Dec . 14(12):993-1000. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8827" in your Materials and Methods section.

Price: US $65

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