Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pEF1a-FB-dCas9-puro Citations (14)

Originally described in: In Situ Capture of Chromatin Interactions by Biotinylated dCas9.
Liu X, Zhang Y, Chen Y, Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J Cell. 2017 Aug 24;170(5):1028-1043.e19. doi: 10.1016/j.cell.2017.08.003.
PubMed Journal

Articles Citing pEF1a-FB-dCas9-puro

Articles
CAPTURE: In Situ Analysis of Chromatin Composition of Endogenous Genomic Loci by Biotinylated dCas9. Liu X, Zhang Y, Chen Y, Li M, Shao Z, Zhang MQ, Xu J. Curr Protoc Mol Biol. 2018 Jul;123(1):e64. doi: 10.1002/cpmb.64. Epub 2018 Jun 19. PubMed
Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9. Liu X, Chen Y, Zhang Y, Liu Y, Liu N, Botten GA, Cao H, Orkin SH, Zhang MQ, Xu J. Genome Biol. 2020 Mar 5;21(1):59. doi: 10.1186/s13059-020-01973-w. PubMed

Associated Plasmids

RNF219/alpha-Catenin/LGALS3 Axis Promotes Hepatocellular Carcinoma Bone Metastasis and Associated Skeletal Complications. Zhang S, Xu Y, Xie C, Ren L, Wu G, Yang M, Wu X, Tang M, Hu Y, Li Z, Yu R, Liao X, Mo S, Wu J, Li M, Song E, Qi Y, Song L, Li J. Adv Sci (Weinh). 2020 Dec 31;8(4):2001961. doi: 10.1002/advs.202001961. eCollection 2021 Feb. PubMed
SIRT1 regulates sphingolipid metabolism and neural differentiation of mouse embryonic stem cells through c-Myc-SMPDL3B. Fan W, Tang S, Fan X, Fang Y, Xu X, Li L, Xu J, Li JL, Wang Z, Li X. Elife. 2021 May 27;10. pii: 67452. doi: 10.7554/eLife.67452. PubMed
Shape of promoter antisense RNAs regulates ligand-induced transcription activation. Yang F, Tanasa B, Micheletti R, Ohgi KA, Aggarwal AK, Rosenfeld MG. Nature. 2021 Jul;595(7867):444-449. doi: 10.1038/s41586-021-03589-x. Epub 2021 Jun 30. PubMed
Circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF. Chen Z, Lu T, Huang L, Wang Z, Yan Z, Guan Y, Hu W, Fan Z, Zhu P. J Clin Invest. 2021 Oct 1;131(19). pii: e148020. doi: 10.1172/JCI148020. PubMed
Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture. Cha HJ, Uyan O, Kai Y, Liu T, Zhu Q, Tothova Z, Botten GA, Xu J, Yuan GC, Dekker J, Orkin SH. Nat Commun. 2021 Oct 29;12(1):6241. doi: 10.1038/s41467-021-26574-4. PubMed
circREEP3 Drives Colorectal Cancer Progression via Activation of FKBP10 Transcription and Restriction of Antitumor Immunity. Chen Z, He L, Zhao L, Zhang G, Wang Z, Zhu P, Liu B. Adv Sci (Weinh). 2022 May;9(13):e2105160. doi: 10.1002/advs.202105160. Epub 2022 Mar 1. PubMed
Chromatin-associated orphan snoRNA regulates DNA damage-mediated differentiation via a non-canonical complex. Han C, Sun LY, Luo XQ, Pan Q, Sun YM, Zeng ZC, Chen TQ, Huang W, Fang K, Wang WT, Chen YQ. Cell Rep. 2022 Mar 29;38(13):110421. doi: 10.1016/j.celrep.2022.110421. PubMed
Gut microbiota drives macrophage-dependent self-renewal of intestinal stem cells via niche enteric serotonergic neurons. Zhu P, Lu T, Wu J, Fan D, Liu B, Zhu X, Guo H, Du Y, Liu F, Tian Y, Fan Z. Cell Res. 2022 Jun;32(6):555-569. doi: 10.1038/s41422-022-00645-7. Epub 2022 Apr 4. PubMed
Chromatin Rewiring by Mismatch Repair Protein MSH2 Alters Cell Adhesion Pathways and Sensitivity to BET Inhibition in Gastric Cancer. Nargund AM, Xu C, Mandoli A, Okabe A, Chen GB, Huang KK, Sheng T, Yao X, Teo JMN, Sundar R, Kok YJ, See YX, Xing M, Li Z, Yong CH, Anand A, A I ZF, Poon LF, Ng MSW, Koh JYP, Ooi WF, Tay ST, Ong X, Tan ALK, Grabsch HI, Fullwood MJ, Teh TB, Bi X, Kaneda A, Li S, Tan P. Cancer Res. 2022 Jul 18;82(14):2538-2551. doi: 10.1158/0008-5472.CAN-21-2072. PubMed
The risk variant rs11836367 contributes to breast cancer onset and metastasis by attenuating Wnt signaling via regulating NTN4 expression. Yang H, Ting X, Geng YH, Xie Y, Nierenberg JL, Huo YF, Zhou YT, Huang Y, Yu YQ, Yu XY, Li XF, Ziv E, Zhang H, Fang WG, Shen Y, Tian XX. Sci Adv. 2022 Jun 10;8(23):eabn3509. doi: 10.1126/sciadv.abn3509. Epub 2022 Jun 10. PubMed
NKX2-8/PTHrP Axis-Mediated Osteoclastogenesis and Bone Metastasis in Breast Cancer. Abudourousuli A, Chen S, Hu Y, Qian W, Liao X, Xu Y, Song L, Zhang S, Li J. Front Oncol. 2022 May 30;12:907000. doi: 10.3389/fonc.2022.907000. eCollection 2022. PubMed
KLF4-mediated upregulation of the NKG2D ligand MICA in acute myeloid leukemia: a novel therapeutic target identified by enChIP. Alkhayer R, Ponath V, Frech M, Adhikary T, Graumann J, Neubauer A, von Strandmann EP. Cell Commun Signal. 2023 May 4;21(1):94. doi: 10.1186/s12964-023-01118-z. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.