pAAV-TREtight-mTagBFP2-B19G
(Plasmid #100799)

Purpose

helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA
Depositing Lab

Ian Wickersham
Publication

Liu et al Nature. 2017 Aug 31;548(7669):582-587. doi: 10.1038/nature23663. Epub 2017 Aug 23. ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	100799	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Discontinued

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pAAV
(Search Vector Database)
Backbone manufacturer
Stratagene
Vector type
Mammalian Expression, AAV

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
Species
Synthetic
Insert Size (bp)
2337
Promoter TREtight

Cloning Information

Cloning method Gibson Cloning
5′ sequencing primer unknown
3′ sequencing primer unknown

(Common Sequencing Primers)

Resource Information

Terms and Licenses
- UBMTA
- Evrogen Limited Use Label License for FPs

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pAAV-TREtight-mTagBFP2-B19G was a gift from Ian Wickersham (Addgene plasmid # 100799)
For your References section:

Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep. Liu K, Kim J, Kim DW, Zhang YS, Bao H, Denaxa M, Lim SA, Kim E, Liu C, Wickersham IR, Pachinis V, Hattar S, Song J, Brown SP, Blackshaw S. Nature. 2017 Aug 31;548(7669):582-587. doi: 10.1038/nature23663. Epub 2017 Aug 23. 10.1038/nature23663 PubMed 28847002

pAAV-TREtight-mTagBFP2-B19G (Plasmid #100799)