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LLP252 pEF1a-NLS-scFvGCN4-DNMT3a
(Plasmid #100941)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100941 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    LLP245 pEF1a-TALE-DNMT3a-Wt
  • Backbone size w/o insert (bp) 4321
  • Total vector size (bp) 7190
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    5157
  • Mutation
    D10A, H840A for dCas9
  • Entrez Gene
    DNMT3A (a.k.a. DNMT3A2, M.HsaIIIA, TBRS)
  • Tags / Fusion Proteins
    • 3xTy1 (C terminal on insert)
    • 1xHA (N terminal on insert)

Resource Information

Depositor Comments

scFvGCN4 domain obtained from pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (Addgene Plasmid #60904)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LLP252 pEF1a-NLS-scFvGCN4-DNMT3a was a gift from Ryan Lister (Addgene plasmid # 100941 ; http://n2t.net/addgene:100941 ; RRID:Addgene_100941)
  • For your References section:

    A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Pflueger C, Tan D, Swain T, Nguyen TV, Pflueger J, Nefzger C, Polo JM, Ford E, Lister R. Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117. 10.1101/gr.233049.117 PubMed 29907613