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pCMV-BE-zCas9
(Plasmid #101739)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101739 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    CMV
  • Backbone manufacturer
    origene
  • Backbone size w/o insert (bp) 3405
  • Total vector size (bp) 8532
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rAPOBEC1-XTEN-zCas9-UGI-NLS
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    5127
  • Mutation
    D10A
  • GenBank ID
  • Promoter CMV

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-BE-zCas9 was a gift from Shuo Lin (Addgene plasmid # 101739)
  • For your References section:

    Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. Zhang Y, Qin W, Lu X, Xu J, Huang H, Bai H, Li S, Lin S. Nat Commun. 2017 Jul 25;8(1):118. doi: 10.1038/s41467-017-00175-6. 10.1038/s41467-017-00175-6 [pii] PubMed 28740134