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pCSN067
(Plasmid #101748)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101748 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS414
  • Total vector size (bp) 11322
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    TRP1 ; KanMX

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
  • Alt name
    LbCpf1
  • Species
    Lachnospiraceae bacterium ND2006
  • Insert Size (bp)
    3720
  • Promoter Heterologous promoter from K. lactis (KLLA0F20031g).
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    KanMX marker expression cassette.
  • Promoter Heterologous TEF1 promoter from A. gossypii.

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer .
  • 3′ sequencing primer .
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSN067 was a gift from Rene Verwaal (Addgene plasmid # 101748 ; http://n2t.net/addgene:101748 ; RRID:Addgene_101748)
  • For your References section:

    CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae. Verwaal R, Buiting-Wiessenhaan N, Dalhuijsen S, Roubos JA. Yeast. 2017 Sep 8. doi: 10.1002/yea.3278. 10.1002/yea.3278 PubMed 28886218