PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||101782||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR ; Donor Template
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameLAMP1 Homology Arms with linker-mEGFP
SpeciesH. sapiens (human)
Insert Size (bp)2777
Entrez GeneLAMP1 (a.k.a. CD107a, LAMPA, LGP120)
/ Fusion Protein
- linker-mEGFP (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human LAMP1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: EFGSTGSTGSTGADPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AICSDP-19:LAMP1-mEGFP was a gift from Allen Institute for Cell Science (Addgene plasmid # 101782 ; http://n2t.net/addgene:101782 ; RRID:Addgene_101782)