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pGL3-mFgf21pro3-luc
(Plasmid #101799)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101799 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fibroblast growth factor 21 promoter
  • Alt name
    Fgf21 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    217
  • Entrez Gene
    Fgf21
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer RVprimer3
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-mFgf21pro3-luc was a gift from Seiichi Oyadomari (Addgene plasmid # 101799 ; http://n2t.net/addgene:101799 ; RRID:Addgene_101799)
  • For your References section:

    Skeletal muscle-specific eukaryotic translation initiation factor 2alpha phosphorylation controls amino acid metabolism and fibroblast growth factor 21-mediated non-cell-autonomous energy metabolism. Miyake M, Nomura A, Ogura A, Takehana K, Kitahara Y, Takahara K, Tsugawa K, Miyamoto C, Miura N, Sato R, Kurahashi K, Harding HP, Oyadomari M, Ron D, Oyadomari S. FASEB J. 2016 Feb;30(2):798-812. doi: 10.1096/fj.15-275990. Epub 2015 Oct 20. 10.1096/fj.15-275990 PubMed 26487695