pLenti Lifeact-mTagBFP2 PuroR
(Plasmid #101893)

• Purpose: Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons
• Depositing Lab: Ghassan Mouneimne
• Publication: Lifeact Plasmids (unpublished)

Backbone

• Vector backbone: pMuLE Dest PuroR
• Backbone size w/o insert (bp): 8690
• Total vector size (bp): 8741
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Lifeact
• Species: Synthetic
• Insert Size (bp): 51
• Promoter: CMV immediate early
• Tag / Fusion Protein:
  • mTagBFP2 (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV_fwd_primer
• 3′ sequencing primer: MSCV_rev_primer

Resource Information

• Supplemental Documents:
  • Datasheet
• A portion of this plasmid was derived from a plasmid made by: Michael Davidson, Addgene #54602
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry