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pLenti Lifeact-mRuby2 BlastR
(Plasmid #84384)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84384 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMuLE Dest BlastR
  • Backbone size w/o insert (bp) 3834
  • Total vector size (bp) 8511
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Lifeact
  • Species
    Synthetic
  • Insert Size (bp)
    51
  • Promoter CMV immediate early
  • Tag / Fusion Protein
    • mRuby2 (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMV_fwd_primer
  • 3′ sequencing primer MSCV_rev_primer
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Michael Davidson, Addgene #54674. Sebastian Zeltzer, University of Arizona.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti Lifeact-mRuby2 BlastR was a gift from Ghassan Mouneimne (Addgene plasmid # 84384 ; http://n2t.net/addgene:84384 ; RRID:Addgene_84384)
  • For your References section:

    High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons. Parker SS, Moutal A, Cai S, Chandrasekaran S, Roman MR, Koshy AA, Khanna R, Zinsmaier KE, Mouneimne G. eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-0135-18. doi: 10.1523/ENEURO.0135-18.2018. eCollection 2018 Sep-Oct. eN-MNT-0135-18 [pii] PubMed 30263951