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pWP1_mFTCD
(Plasmid #102317)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102317 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ftcd
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1626
  • Entrez Gene
    Ftcd
  • Promoter EF1alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PME1 (unknown if destroyed)
  • 3′ cloning site PME1 (unknown if destroyed)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWP1_mFTCD was a gift from David Sabatini (Addgene plasmid # 102317 ; http://n2t.net/addgene:102317 ; RRID:Addgene_102317)
  • For your References section:

    Histidine catabolism is a major determinant of methotrexate sensitivity. Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM. Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 10.1038/s41586-018-0316-7. 10.1038/s41586-018-0316-7 PubMed 29995852