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pLKO-puro FLAG SREBP2
(Plasmid #32018)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pLKO.1-puro
  • Backbone size w/o insert (bp) 7100
  • Modifications to backbone
    U6 replaced by CMV and FLAG added N-term to insert. 5->3': CMV-> FLAG -> sal I restriction site -> 1 base(to keep in frame) -> cDNA -> Not I restriction site
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    SREBP2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Srebf2 (a.k.a. AI608257, SREBP-2, SREBP2, SREBP2gc, bHLHd2, lop13, nuc)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV forward
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO-puro FLAG SREBP2 was a gift from David Sabatini (Addgene plasmid # 32018)
  • For your References section:

    mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM. Cell. 2011 Aug 5;146(3):408-20. 10.1016/j.cell.2011.06.034 PubMed 21816276