1
Plasmid 32018: pLKO-puro FLAG SREBP2
  • SREBP2

  • M. musculus (mouse)

  • Srebf2 (AI608257, SREBP-2, SREBP2, SREBP2gc, bHLHd2, lop13, nuc)

  • FLAG

  • N terminal on backbone

  • pLKO.1-puro
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 7100

  • U6 replaced by CMV and FLAG added N-term to insert. 5->3': CMV-> FLAG -> sal I restriction site -> 1 base(to keep in frame) -> cDNA -> Not I restriction site

  • CMV

  • SalI

  • No

  • NotI

  • No

  • CMV forward List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • Puromycin

  • View sequences (1)
  • David Sabatini

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson et al (Cell. 2011 Aug 5;146(3):408-20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32018" in your Materials and Methods section.