pLKO-puro FLAG SREBP2
- Backbone size w/o insert (bp) 7100
Modifications to backboneU6 replaced by CMV and FLAG added N-term to insert. 5->3': CMV-> FLAG -> sal I restriction site -> 1 base(to keep in frame) -> cDNA -> Not I restriction site
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
SpeciesM. musculus (mouse)
Entrez GeneSrebf2 (a.k.a. AI608257, SREBP-2, SREBP2, SREBP2gc, bHLHd2, lop13, nuc)
- Promoter CMV
/ Fusion Protein
- FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO-puro FLAG SREBP2 was a gift from David Sabatini (Addgene plasmid # 32018)
For your References section:mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM. Cell. 2011 Aug 5;146(3):408-20. 10.1016/j.cell.2011.06.034 PubMed 21816276
Map generated by Addgene from plasmid data.