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pcDNA3.1-hNurr1-NE
(Plasmid #102363)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102363 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NR4A2 nuclear receptor subfamily 4 group A member 2
  • Alt name
    Nurr1
  • Alt name
    NOT; RNR1; HZF-3; TINUR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1851
  • Entrez Gene
    NR4A2 (a.k.a. HZF-3, NOT, NURR1, RNR1, TINUR)
  • Promoter CMV
  • Tag / Fusion Protein
    • NE (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7 primer/CMV-F primer
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

This plasmid encodes a novel 18-amino-acid protein tag - "NE", which can be detected by a specific mouse monoclonal antibody in Western blotting, immunopreciptation, and immunocytochemistry. (Source of antibody: http://www.versitech.hku.hk/reagents/ne/)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-hNurr1-NE was a gift from Shu Leong Ho (Addgene plasmid # 102363 ; http://n2t.net/addgene:102363 ; RRID:Addgene_102363)