Addgene - OKSIM Plasmid Data

Plasmid 24603: OKSIM

Gene/insert name:
Oct4 KLF4 Sox2 c-Myc
Insert size:
5600
Species:
H. sapiens (human)
Entrez Gene:
SOX2 (ANOP3, MCOPS3)
POU5F1 (DADB-104B20.2, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
MYC (MRTL, bHLHe39, c-Myc)
KLF4 (RP11-150J11.1, EZF, GKLF)
Mutation:
Oct4-2A-KLF4-2A-Sox2-IRES-Myc
Vector backbone:
pSin4-EF2-IRES-Pur
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, Cre/Lox
Backbone size w/o insert (bp):
6777
Cloning site 5':
Mlu I
Site destroyed during cloning:
No
Cloning site 3':
Pme I
Site destroyed during cloning:
No
5' sequencing primer:
EF-1a Forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
The pSin4-EF2-IRES-Pur was obtained from Addgene (James Thomson's laboratory) in the form of pSin-EF2-Oct4-Pur. The WPRE/LoxP LTR portion was obtained from Addgene in the form of pWPT-GFP (Luigi Naldini's laboratory). Transgene ORFs were cloned from human factor cDNAs.
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Jose Cibelli
Terms and Licenses:
MTA

Comments:

Constructed by Steven T. Suhr, MSU Cellular Reprogramming Laboratory

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Human Induced Pluripotent Stem Cells Produced Under Xeno-Free Conditions. Ross et al (Stem Cells Dev. 2009 Dec 23. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24603" in your Materials and Methods section.