The pSin4-EF2-IRES-Pur was obtained from Addgene (James Thomson's laboratory) in the form of pSin-EF2-Oct4-Pur. The WPRE/LoxP LTR portion was obtained from Addgene in the form of pWPT-GFP (Luigi Naldini's laboratory). Transgene ORFs were cloned from human factor cDNAs.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
24603" in your Materials and Methods section.