Addgene - pKP332 (Lenti-OSK1) Plasmid Data

Plasmid 21627: pKP332 (Lenti-OSK1)

Gene/insert name:
hOCT4 - 2A - hSOX2 - 2A - hKLF4
Alt name:
POU5F1
Alt name:
Porcine teschovirus-1 2A peptide
Insert size:
3589
Species:
H. sapiens (human)
Species:
Porcine teschovirus-1
GenBank ID:
BC117435 BC029923 BC013923
Entrez Gene:
POU5F1 (DADB-104B20.2, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
Vector backbone:
pDL171 (pWPI with deletion of 1409 bp XhoI [EMCV IRES GFP] frag)
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, Cre/Lox
Backbone size w/o insert (bp):
9692
Cloning site 5':
SwaI
Site destroyed during cloning:
No
Cloning site 3':
SwaI
Site destroyed during cloning:
No
5' sequencing primer:
GGCACCTCGATTAGTTCTCGAGC List of Sequencing Primers
3' sequencing primer:
AATCCAGAGGTTGATTATCG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (6)
Principal Investigator:
Tim Townes
Terms and Licenses:
MTA

Comments:

Plasmid constructed by Kevin Pawlik

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Polycistronic lentiviral vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Chang et al (Stem Cells. 2009 May . 27(5):1042-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21627" in your Materials and Methods section.