pcDNA-f:PGC1b
(Plasmid #1031)

• Depositing Lab: Bruce Spiegelman
• Publication: Lin et al J Biol Chem 2002 Jan 18;277(3):1645-8. Epub 2001 Nov 30.

Backbone

• Vector backbone: pcDNA-flag
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PGC-1 beta
• Alt name: PGC1beta
• Alt name: PGC-1b
• Alt name: PPAR gamma coactivator 1 beta
• Species: M. musculus (mouse)
• Insert Size (bp): 3660
• GenBank ID: NM_133249
• Entrez Gene: Ppargc1b (a.k.a. 4631412G21Rik, PGC-1beta, PGC-1beta/ERRL1, PPARGC-1-beta, Perc)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry