PurposePlasmid encoding 2ndVal mKate2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||104030||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3224
- Total vector size (bp) 3923
Modifications to backboneSee "Notes On Plasmids In This Collection"
Vector typeBacterial Expression ; Low copy
Growth in Bacteria
Copy numberLow Copy
Gene/Insert name2ndVal mKate2
Insert Size (bp)699
MutationmKate2 with valine inserted in 2nd position
- Promoter proC
- Cloning method Gibson Cloning
- 5′ sequencing primer GCGTATCACGAGGCCCTTTC
- 3′ sequencing primer AAAGGGAAAACTGTCCATATGCAC (Common Sequencing Primers)
This is a version of mKate2 with a valine inserted in the second position. Please see "Notes On Plasmids In This Collection" document for further details and references.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEB2-2ndVal mKate2 was a gift from Philippe Cluzel (Addgene plasmid # 104030)
For your References section:Systematic characterization of maturation time of fluorescent proteins in living cells. Balleza E, Kim JM, Cluzel P. Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 2017 Nov 20. 10.1038/nmeth.4509 PubMed 29320486