Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #104174)


Item Catalog # Description Quantity Price (USD)
Plasmid 104174 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositing lab has noted that the yield of plasmid after mini prep could be low due to a discrepancy in the ori sequence. The differences in sequence do not affect function of this plasmid for mammalian expression of sgRNA,

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRG2 was a gift from Jin-Soo Kim (Addgene plasmid # 104174 ; ; RRID:Addgene_104174)
  • For your References section:

    Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides. Kim S, Bae T, Hwang J, Kim JS. Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3. 10.1186/s13059-017-1355-3 [pii] PubMed 29141659