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pRG2
(Plasmid #104174)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104174 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Depositor Comments

The depositing lab has noted that the yield of plasmid after mini prep could be low due to a discrepancy in the ori sequence. The differences in sequence do not affect function of this plasmid for mammalian expression of sgRNA,

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRG2 was a gift from Jin-Soo Kim (Addgene plasmid # 104174 ; http://n2t.net/addgene:104174 ; RRID:Addgene_104174)
  • For your References section:

    Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides. Kim S, Bae T, Hwang J, Kim JS. Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3. 10.1186/s13059-017-1355-3 [pii] PubMed 29141659