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pG-HIV-LRT
(Plasmid #104592)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104592 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGemT
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3001
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIV-1 late reverse transcripts
  • Species
    HIV-1 virus

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer T7-F TAATACGACTCACTATAGGG and SP6-F ATTTAGGTGACACTATAG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

This plasmid may be used as a qPCR standard for the measure of HIV-1 virus. It encodes a short fragment of the HIV-1 genome corresponding to 557-699 bp in the HXB2 reference strain sequence. This sequence corresponds to 674-816 bp in reverse in the Addgene sequence file. It may be amplified with primers MH531 (5' TGTGTGCCCGTCTGTTGTGT), MH532 (5' GAGTCCTGCGTCGAGAGAGC), and Taqman probe LRT-P (5' CAGTGGCGCCCGAACAGGGA)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pG-HIV-LRT was a gift from Kristine Yoder (Addgene plasmid # 104592 ; http://n2t.net/addgene:104592 ; RRID:Addgene_104592)
  • For your References section:

    Real-time quantitative PCR and fast QPCR have similar sensitivity and accuracy with HIV cDNA late reverse transcripts and 2-LTR circles. Yoder KE, Fishel R. J Virol Methods. 2008 Nov;153(2):253-6. doi: 10.1016/j.jviromet.2008.07.032. Epub 2008 Sep 17. 10.1016/j.jviromet.2008.07.032 PubMed 18762215