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BB3cK_pGAP_23*_pTEF_Cas9
(Plasmid #104909)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104909 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Episomal ARS/CEN Backbone
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    HH - FS23 linker - HDV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (not destroyed)
  • 3′ cloning site BbsI (not destroyed)
  • 5′ sequencing primer pGAP fw
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
  • Alt name
    hcas9
  • Species
    H. sapiens (human); Streptococcus pyogenes, Pichia pastoris
  • Insert Size (bp)
    4140

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The original Cas9 sequence was taken from from DiCarlo et al., (Addgene ID 43802). Reference: DiCarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM. 2013. Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res 41:4336-43.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Additional Publication: Prielhofer, R. etal (2017) BMC Syst Biol. 11,123 (PMID:29221460)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BB3cK_pGAP_23*_pTEF_Cas9 was a gift from Brigitte Gasser (Addgene plasmid # 104909 ; http://n2t.net/addgene:104909 ; RRID:Addgene_104909)