CRISPR/Cas9-Mediated Homology-Directed Genome Editing in Pichia pastoris.
Gassler T, Heistinger L, Mattanovich D, Gasser B, Prielhofer R
Methods Mol Biol. 2019;1923:211-225. doi: 10.1007/978-1-4939-9024-5_9. PubMed Article
Gassler T, Heistinger L, Mattanovich D, Gasser B, Prielhofer R
Methods Mol Biol. 2019;1923:211-225. doi: 10.1007/978-1-4939-9024-5_9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104906 | BB3cN_pGAP_23*_pLAT1_Cas9 | hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC |
| 104907 | BB3cH_pGAP_23*_pLAT1_Cas9 | hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg |
| 104908 | BB3cK_pGAP_23*_pLAT1_Cas9 | hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418 |
| 104909 | BB3cK_pGAP_23*_pTEF_Cas9 | hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection |
| 104910 | BB3cK_pGAP_23*_pPFK300_Cas9 | hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418 |
| 104911 | BB3cN_pGAP_23*_pPFK300_Cas9 | hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC |
| 104912 | BB3cH_pGAP_23*_pPFK300_Cas9 | hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg |
| 108686 | BB3nK_ext_AC | Plasmid for assembly of donor DNA templates consisting of two expression units |
| 108687 | BB3nK_ext_AD | Plasmid for assembly of donor DNA templates consisting of three expression units |