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pAAV-Ef1a-fDIO-GCaMP6s
(Plasmid #105714)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105714 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-Ef1a-fDIO hChR2(H134R)-EYFP
  • Backbone manufacturer
    Karl Deisseroth
  • Backbone size w/o insert (bp) 5609
  • Total vector size (bp) 6857
  • Vector type
    Mammalian Expression, AAV ; Flp/Frt

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP6s
  • Alt name
    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G
  • Alt name
    GCaMP3 variant 641
  • Species
    R. norvegicus (rat); A. victoria (jellyfish)
  • Insert Size (bp)
    1274
  • Promoter Ef1a, Human elongation factor-1 alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer cCTATTCTTCaAAAGGTATAGGAACTTC
  • 3′ sequencing primer tTCTAGAGAATAGGAACT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Ef1a-fDIO-GCaMP6s was a gift from Rylan Larsen (Addgene plasmid # 105714 ; http://n2t.net/addgene:105714 ; RRID:Addgene_105714)