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pNEU-hMbPylRS-4xU6M15
(Plasmid #105830)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105830 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Humanized MbPylRS
  • Species
    Synthetic; M. barkeri
  • Mutation
    (Y271A, Y349F)MbPylRS
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    tRNAM15
  • Species
    Synthetic
  • Promoter U6

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The codon-optimized gene of MbPylRSF was custom synthesized by GeneArt (ThermoFisher Scientific).
  • Terms and Licenses

Depositor Comments

The plasmid contains a novel tRNA that boosts the efficiency of the Pyrrolysyl system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNEU-hMbPylRS-4xU6M15 was a gift from Irene Coin (Addgene plasmid # 105830 ; http://n2t.net/addgene:105830 ; RRID:Addgene_105830)
  • For your References section:

    Designer tRNAs for efficient incorporation of non-canonical amino acids by the pyrrolysine system in mammalian cells. Serfling R, Lorenz C, Etzel M, Schicht G, Bottke T, Morl M, Coin I. Nucleic Acids Res. 2017 Nov 21. pii: 4641905. doi: 10.1093/nar/gkx1156. 10.1093/nar/gkx1156 PubMed 29177436