PurposePlasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||105830||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameHumanized MbPylRS
SpeciesSynthetic; M. barkeri
- Promoter CMV
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
- Promoter U6
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
The plasmid contains a novel tRNA that boosts the efficiency of the Pyrrolysyl system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNEU-hMbPylRS-4xU6M15 was a gift from Irene Coin (Addgene plasmid # 105830 ; http://n2t.net/addgene:105830 ; RRID:Addgene_105830)
For your References section:Designer tRNAs for efficient incorporation of non-canonical amino acids by the pyrrolysine system in mammalian cells. Serfling R, Lorenz C, Etzel M, Schicht G, Bottke T, Morl M, Coin I. Nucleic Acids Res. 2017 Nov 21. pii: 4641905. doi: 10.1093/nar/gkx1156. 10.1093/nar/gkx1156 PubMed 29177436