pMK231 (AAVS1 CMV-MCS-PURO)
Purpose(Empty Backbone) Donor for integration at the human AAVS1 locus
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||105924||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7873
Vector typeMammalian Expression, CRISPR
- Promoter CMV
Growth in Bacteria
Copy numberHigh Copy
Clone your gene of interest at MCS and integrate it at the human AAVS1 locus using AAVS1 T2 CRIPR in pX330 (Addgene 72833).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMK231 (AAVS1 CMV-MCS-PURO) was a gift from Masato Kanemaki (Addgene plasmid # 105924 ; http://n2t.net/addgene:105924 ; RRID:Addgene_105924)
For your References section:Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T. Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559. 10.7554/eLife.36559 PubMed 29848445