|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12168||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerP. Soriano
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRosa26 CreER^T2
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Targeting vector to generate R26-CreER^T2 mice. XbaI/MluI SA-CreER^T2 fragment of pMB61, MluI/XbaI PA-floxed PGK-Puro fragment of pMB75, and XbaI-digested modified version of pROSA26-1 vector where SacII site is replaced by an AscI linearization site.
See sequence link for sequence of insert from author. Please note that there are minor errors in the sequence, specifically there are no XbaI sites within the insert. Please see Addgene test digest with XbaI for the expected bands.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMB80 (R26-CreER) was a gift from Tyler Jacks (Addgene plasmid # 12168 ; http://n2t.net/addgene:12168 ; RRID:Addgene_12168)
For your References section:The Nf2 tumor suppressor regulates cell-cell adhesion during tissue fusion. McLaughlin ME, Kruger GM, Slocum KL, Crowley D, Michaud NA, Huang J, Magendantz M, Jacks T. Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3261-6. 10.1073/pnas.0700044104 PubMed 17360635