SunTagng14aa
(Plasmid #106439)

• Purpose: Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
• Depositing Lab: Steven Jacobsen
• Publication: Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 10.1073/pnas.1716945115. Epub 2018 Feb 14.

Backbone

• Vector backbone: pMOA34
• Vector type: Plant Expression, CRISPR, Synthetic Biology
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
• Alt name: SunTagng-14aa
• Species: H. sapiens (human), A. thaliana (mustard weed), Synthetic
• Insert Size (bp): 16290

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Portions of the SunTagng-14aa-TET1cd were amplified from Addgene plasmid # 60903 and # 60904, gifts from Ron Vale. The pMOA34 backbone was a gift from Zachary Nimchuk.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry