|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||106477||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 12951
Modifications to backbonereplaced EGFP with Blasticidin resistance gene
Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter EFS
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Gibson Cloning
A portion of this plasmid was derived from a plasmid made byBlasticidin resistance gene from Feng Zhang lab's lentiCas9-Blast plasmid (Addgene#52962); backbone originally derived from the CROPseq-Guide construct from Christopher Bock's Lab (Addgene#86708)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CROPseq-EFS-SpCas9-P2A-blast was a gift from Alex Hewitt (Addgene plasmid # 106477 ; http://n2t.net/addgene:106477 ; RRID:Addgene_106477)