|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||106965||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5280
- Total vector size (bp) 13704
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesSynthetic; Streptococcus pyogenes
Insert Size (bp)4212
- Promoter EFS
- Cloning method Gibson Cloning
- 5′ sequencing primer ACGGGTTTGCCGCCAGAACA (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byHypaSpCas9 cloned from BPK4410 plasmid (Addgene#101178) from Jennifer Doudna and Keith Joung labs. CROPseq-Guide-EFS-SpCas9-P2A-Puro backbone used to make this construct was originally derived from the CROPseq-Guide-Puro (Addgene#86708) from Christoph Bock Lab.
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CROPseq-EFS-HypaSpCas9-P2A-puro was a gift from Alex Hewitt (Addgene plasmid # 106965 ; http://n2t.net/addgene:106965 ; RRID:Addgene_106965)